ENU MUTAGENESIS IN THE MOUSE ELECTROPHORETIC SPECIFIC-LOCUS TEST 2. MUTATIONAL STUDIES OF MATURE OOCYTES

Abstract

Experiments were conducted using the biochemical specific locus test to assess the mutagenicity of N-ethyl-N-nitrosourea (ENU) in mature oocytes of mice. 57Bl/6J females were treated with 100 mg/kg ENU by intraperitoneal injection and mated to untreated DBA males for one week following treatment. 1,447 progeny were screened for evidence of mutations affecting the electrophoretic mobility of 32 different proteins; two mutants were detected by electrophoretic analyses. hese results provide evidence that ENU is a germ cell mutagen in mouse oocytes, although the frequency of mutants is somewhat lower than that obtained from spermatogonia treated with the same dose.

Citation

Lewis, S. ENU MUTAGENESIS IN THE MOUSE ELECTROPHORETIC SPECIFIC-LOCUS TEST 2. MUTATIONAL STUDIES OF MATURE OOCYTES. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-93/178.

Additional Information

Mutation Research 296(1/2):129-133, 1992

• ENU MUTAGENESIS IN THE MOUSE ELECTROPHORETIC SPECIFIC-LOCUS TEST 2. MUTATIONAL STUDIES OF MATURE OOCYTES