Method: Culture of 16HBE14o- Cells

This protocol describes the thawing, culturing, and cryopreservation of the human bronchial epithelial cell line 16HBE14o- (referred to as “16HBE”). Disclaimer: The contents of this article have been reviewed by the US Environmental Protection Agency and approved for publication and do not necessarily represent Agency policy. Mention of trade names or commercial products does not constitute endorsement or recommendations for use.

Impact/Purpose

This method is being made publicly available via Nature Protocol Exchange to support published EPA research and ensure transparency and reproducibility.

Citation

McCullough, S. Method: Culture of 16HBE14o- Cells. Springer Nature, LONDON, UK, 2020. [DOI: 10.21203/rs.2.20353/v1]

DOI: Method: Culture of 16HBE14o- Cells