Culture of NCI-H441 Cells in Advanced RPMI

This protocol describes the thawing, culturing, and cryopreservation of the human alveolar-like epithelial cell line NCI-H441. NCI-H441 cells should be used in place of the adenocarcinoma cell line, A549, when studying the human alveolar epithelium as, in contrast to A549 cells, H441 cells reflect the in vivo alveolar epithelium barrier and transporter protein function. 

Impact/Purpose

This protocol describes methods used for the culture of the NCI-H441 human cell line.

Citation

Vitucci, E. AND S. McCullough. Culture of NCI-H441 Cells in Advanced RPMI. Springer Nature, LONDON, UK1, (2022). [DOI: 10.21203/rs.3.pex-2011/v1]

DOI: Culture of NCI-H441 Cells in Advanced RPMI