Measurement of trans-epithelial electrical resistance with EndOhm cup and EVOM2

Trans-epithelial Electrical Resistance (TEER) can be used as a measure of cell monolayer confluence, health, and integrity.  An EndOhm chamber connected to an Epithelial Voltohmmeter (EVOM) may be used to take TEER measurements that are more reproducible than the chopstick electrode.  This method details how to take TEER readings using an EndOhm chamber and EVOM2 manufactured by World Precision Instruments.

Impact/Purpose

This is a revised protocol for measuring trans-epithelial electrical resistance for in vitro respiratory epithelial cell systems. Making this protocol publicly accessible through the Nature Research Protocol Exchange will support the transparency and reproducibility inhaled chemical research and testing within the EPA and in the field at-large.

Citation

Mallek, N. AND S. McCullough. Measurement of trans-epithelial electrical resistance with EndOhm cup and EVOM2. Springer Nature, LONDON, UK1, (2021). [DOI: 10.21203/rs.3.pex-1672/v1]

DOI: Measurement of trans-epithelial electrical resistance with EndOhm cup and EVOM2