Biosyper Transcriptomic Assessment of Fetal and Neonatal Brain from Thyroid Hormone Disruption
Gene profiles in animals exposed to two prototypic thyroid hormone disrupting chemicals, one acting to inhibit thyroid hormone synthesis by inhibiting the enzyme thyroperoxidase (TPO), the other that blocks iodine uptake into the thyroid gland by inhibiting the sodium iodine symporter (NIS). Forebrain regions of fetal and newborn rats born to dams exposed to propylthiouracil or perchlorate were collected on gestational day 20, postnatal day 0, or postnatal day 2. Samples collected from offspring of dams exposed to the TPO inhibitor propylthiouracil or the NIS inhibitor perchlorate will be subjected a TempO-Seq® whole-genome targeted sequencing assay. This transcriptomic analysis in at these early ages will identify gene targets that may prove useful in detection and characterization of thyroid hormone dependent impairments in brain development and serve as biomarkers of effect. The goal is to discover novel biomarkers of effect that could be used in assessing health hazards imposed by chemical-induced reductions in thyroid hormone during pregnancy.