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Using genus-level taxonomy and traits to develop diatom multimetric indices to assess wetland condition across the conterminous United States

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In large-extent assessment programs, such as the United States Environmental Protection Agency's (USEPA) National Wetland Condition Assessment (NWCA), the task of identifying diatom taxa in samples is spread out over several taxonomists and laboratories. This produces inconsistencies in the diatom data at the species-level that have proven difficult to reconcile. New approaches for using genus-level diatom data, and assigning traits to each genus, have shown promise for introducing diatoms into national assessments in rivers and streams. We developed diatom multimetric indices (MMIs) using genus-level taxonomy and trait information from the NWCA data to assess the ecological condition for 574 inland and estuarine wetland samples across the conterminous U.S. Genus-level, trait-based indices have several advantages over classic species-based indices, including less work-effort and expertise required. Using genus-level diatom data is also more reliable for bioassessment when there are data quality concerns because of taxonomic inconsistencies from using multiple analysts. Our study showed that the genus-level, trait MMIs responded well to increases in multiple stressors, and discriminated least-disturbed from most-disturbed wetland sites. Inland wetland MMIs discriminated better than the estuarine MMI. The performance of our MMIs was similar to that of other existing USEPA national aquatic monitoring MMIs, including the wetland vegetation MMI. This new indicator may allow for the use of diatoms in NWCA surveys, and our cost-effective approach may facilitate the use of diatoms in other aquatic programs with restricted resources. Moreover, our approach could be applied to older data previously set aside due to taxonomic challenges.

Impact/Purpose

For large-scale and long-term assessment programs that require multiple taxonomic laboratories to process samples, such as the United States Environmental Protection Agency’s (U.S. EPA’s) National Wetland Condition Assessment (NWCA), discrepancies in species-level identification or nomenclature has prevented the use of diatom data in regional and national assessments. To address this problem, EPA-ORD-PESD Luisa Riato and Amanda Nahlik, and Alan Herlihy (Oregon State University), developed diatom multimetric indices (MMIs) using genus-level diatom taxonomy and trait-based autecological information, effectively circumventing the problem of taxonomic inconsistencies in the NWCA diatom datasets from 2011. In contrast to traditional species-level approaches, trait-based approaches can use genus-level data, which is simpler and less-expensive to obtain, and can eliminate discrepancies in species-level identification or nomenclature that can reduce confidence and defensibility of the datasets. The MMIs are designed to assess wetland ecological condition in five large ecoregions across the conterminous United States - Eastern Mountains and Upper Midwest (East), Interior Plains (Plains), Inland Coastal Plains (ICP), West (West), and the Eastern Estuarine (EST) region. Inland wetland MMIs for the East, Plains, ICP, and West discriminated better than the estuarine (EST) MMI. The performance of the MMIs was comparable to that of other existing EPA national aquatic monitoring MMIs including the wetland vegetation MMI. The authors demonstrate that trait-based MMIs can be effective for large-extent assessments, and that using a lower taxonomic resolution could lead to substantial savings for large regional and national assessments. This cost-effective approach also facilitates including of diatoms into regional/state/tribe assessments with limited resources.

Citation

Riato, L., A. Herlihy, AND A. Nahlik. Using genus-level taxonomy and traits to develop diatom multimetric indices to assess wetland condition across the conterminous United States. Elsevier BV, AMSTERDAM, NETHERLANDS, 1028:181720, (2026). [DOI: 10.1016/j.scitotenv.2026.181720]

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DOI: Using genus-level taxonomy and traits to develop diatom multimetric indices to assess wetland condition across the conterminous United States
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Last updated on March 23, 2026
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