SPATIAL DISTRIBUTION OF SPERM-DERIVED CHROMATIN IN ZYGOTES DETERMINED BY FLUORESCENCE IN SITU HYBRIDIZATION

Abstract

Fluorescence in situ hybridization was used to determine the spatial distribution of chromatin in zygote pronuclei. ybrid system involving golden hamster eggs and individual human sperm permitted use of DNA probes specific for the entire human chromosome 4, for the heterochromatic region on the long arm of the human Y chromosome and for unique DNA sequences on human chromosome 19. hromosome 4 occupied a circumscribed domain in the pronuclei, similar to findings in somatic interphases. nlike the situation in somatic interphases, the Y heterochromatin was extended throughout the first cell cycle. ronuclear chromatin was extended 3- 4- fold compared to somatic interphase chromatin. he extended pronuclear chromatin conformation is likely to affect a zygote's susceptibility to environmental hazards.

Citation

Brandriff, B. AND L. Gordon. SPATIAL DISTRIBUTION OF SPERM-DERIVED CHROMATIN IN ZYGOTES DETERMINED BY FLUORESCENCE IN SITU HYBRIDIZATION. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-93/172.

Additional Information

Mutation Research 296(1/2):33-42, 1992

• SPATIAL DISTRIBUTION OF SPERM-DERIVED CHROMATIN IN ZYGOTES DETERMINED BY FLUORESCENCE IN SITU HYBRIDIZATION