MUTAGENICITY OF 7H-DIBENZO(C,G)CARBAZOLE AND METABOLITES IN SALMONELLA TYPHIMURIUM

Abstract

7H-Dibenzo(c,g)carbazole (DBC) is a potent carcinogen of environmental import. Reverse-mutation plate-incorporation assays for mutagenicity were undertaken in Salmonella typhimurium strains TA98 and TA100. esults were negative when no exogenous activation system was used, as well as when assays incorporated liver homogenates (S9) from rats, mice and rabbits. y contrast DBC was mutagenic in a forward mutation assay in Salmonella strain TM677 using resistance to 8-azaguanine for selection. etabolites of DBC were generated by incubation with rat-liver microsomes and separated by HPLC. wo of these metabolites were directly mutagenic for Salmonella strain TM677 while two others mutagenic upon addition of S9. Synthetic phenolic derivatives of DBC were also mutagenic in this assay when further metabolized. t is likely that metabolites of DBC phenols constitute the biologically active forms.

Citation

Schoeny, R. AND D. Warshawsky. MUTAGENICITY OF 7H-DIBENZO(C,G)CARBAZOLE AND METABOLITES IN SALMONELLA TYPHIMURIUM. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-87/515 (NTIS PB90242355).

Additional Information

Mutation Research 188:275-286, 1987

• MUTAGENICITY OF 7H-DIBENZO(C,G)CARBAZOLE AND METABOLITES IN SALMONELLA TYPHIMURIUM