Culture of Primary Human Tracheobronchial Epithelial Cells

This protocol is intended for culture of primary human tracheobronchial epithelial cells (pHBEC) obtained by brush biopsy during clinical bronchoscopy or purchased commercially. Note: This is a historical protocol. At the time of publication, this protocol has been superseded by a different version in the McCullough lab; however, it is being published to support the transparency and reproducibility of other studies by which it is referenced. Disclaimer: The information presented here has been reviewed and approved for publication by the US Environmental Protection Agency does not necessarily represent Agency policy. Mention of trade names or commercial products does not constitute endorsement or recommendations for use.

Impact/Purpose

This method is made publicly available via Nature Protocol Exchange to support published EPA research and ensure transparency and reproducibility.

Citation

Dailey, L. AND S. McCullough. Culture of Primary Human Tracheobronchial Epithelial Cells. Nature Publishing Group, London, UK, 2021. [DOI: 10.21203/rs.3.pex-1379/v1]

DOI: Culture of Primary Human Tracheobronchial Epithelial Cells