Measurement of Trans-Epithelial Electrical Resistance with EVOM2 and EndOhm Cup

Trans-epithelial Electrical Resistance (TEER) can be used as a measure of cell monolayer confluence, health, and integrity.  An EndOhm chamber connected to an Epithelial Voltohmmeter (EVOM) may be used to take TEER measurements that are more reproducible than the chopsticks electrode.  This method details how to take TEER readings using an EndOhm-12 chamber and EVOM2 resistance reader.  Disclaimer: The information presented here has been reviewed and approved for publication by the US Environmental Protection Agency does not necessarily represent Agency policy. Mention of trade names or commercial products does not constitute endorsement or recommendations for use.

Impact/Purpose

Trans-epithelial Electrical Resistance (TEER) can be used as a measure of cell monolayer confluence, health, and integrity. This method is being made publicly available via Nature Protocol Exchange to support published EPA research and ensure transparency and reproducibility.   Trans-epithelial Electrical Resistance (TEER) can be used as a measure of cell monolayer confluence, health, and integrity.  An EndOhm chamber connected to an Epithelial Voltohmmeter (EVOM) may be used to take TEER measurements that are more reproducible than the chopsticks electrode.  This method details how to take TEER readings using an EndOhm-12 chamber and EVOM2 resistance reader.  

Citation

Mallek, N. AND S. McCullough. Measurement of Trans-Epithelial Electrical Resistance with EVOM2 and EndOhm Cup. Nature Publishing Group, London, UK, 2021. [DOI: 10.21203/rs.3.pex-1672/v1]

DOI: Measurement of Trans-Epithelial Electrical Resistance with EVOM2 and EndOhm Cup