Culture of IMR90 Cells in Advanced MEM with Reduced Serum

This method describes the thawing, culturing, and cryopreservation of the human lung fibroblast cell line IMR90 in Advanced MEM-based growth medium.  Disclaimer: The contents of this article have been reviewed by the US Environmental Protection Agency and approved for publication and do not necessarily represent Agency policy.  Mention of trade names or commercial products does not constitute endorsement or recommendations for use. 

Impact/Purpose

This method describes the thawing, culturing, and cryopreservation of the human lung fibroblast cell line IMR90 in Advanced MEM-based growth medium.  This method is being made publicly available via Nature Protocol Exchange to support published EPA research and ensure transparency and reproducibility.

Citation

Mallek, N. AND S. McCullough. Culture of IMR90 Cells in Advanced MEM with Reduced Serum. Nature Publishing Group, London, UK, 2021. [DOI: 10.21203/rs.3.pex-1673/v1]

DOI: Culture of IMR90 Cells in Advanced MEM with Reduced Serum