Development of an aerosol compatible cell culture exposure system and its application to quantify cellular uptake of particles at the air liquid interface

• Medium-throughput: 6 doses + 4 technical replicates within standard 24-well cell culture plate • Allows Benchmark Dose (BMD) modeling to estimate in vitro Points of Departure (PODs) for portal of entry effects • Real-time sampling allows accurate exposure conditions to be reported throughout 2 h exposure

Impact/Purpose

Webinar Presentation  The Inhalation Toxicology Facilities Branch (ITFB) developed the EPA’s Cell Culture Exposure Systems (CCES) which permits dynamic exposure of human lung cells to VOCs at the air-liquid interface (ALI).

Citation

Murray, J. Development of an aerosol compatible cell culture exposure system and its application to quantify cellular uptake of particles at the air liquid interface. Association of Inhalation Toxicologists (AIT), Virtual, NC, November 29, 2022.