Method: LIVE/DEAD Fixable Near-IR Cell Viability Assay

This protocol is used to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation. Disclaimer: The contents of this article have been reviewed by the US Environmental Protection Agency and approved for publication and do not necessarily represent Agency policy. Mention of trade names or commercial products does not constitute endorsement or recommendations for use.

Impact/Purpose

This method is being made publicly available via Nature Protocol Exchange to support published EPA research and ensure transparency and reproducibility

Citation

McCullough, S. Method: LIVE/DEAD Fixable Near-IR Cell Viability Assay. Nature Publishing Group, London, UK, 2020. [DOI: 10.21203/rs.2.20493/v1]

DOI: Method: LIVE/DEAD Fixable Near-IR Cell Viability Assay