Method: FITC-Dextran Trans-Epithelial Permeability Assay

This protocol describes the use of the Fluorescein isothiocyanate (FITC)–dextran trans-epithelial permeability assay to quantify the effects of chemicals on epithelial cell monolayer permeability. Disclaimer: The contents of this article have been reviewed by the US Environmental Protection Agency and approved for publication and do not necessarily represent Agency policy. Mention of trade names or commercial products does not constitute endorsement or recommendations for use.

Impact/Purpose

This method is being made publicly available via Nature Protocol Exchange to support published EPA research and ensure transparency and reproducibility.

Citation

McCullough, S. AND S. Farber. Method: FITC-Dextran Trans-Epithelial Permeability Assay. Nature Publishing, 2020. [DOI: 10.21203/rs.2.20495/v1]

DOI: Method: FITC-Dextran Trans-Epithelial Permeability Assay