Method for Tran-Epithelial Exposure Model (TEEM) - Epithelial Cells and Fibroblasts

This exposure model is meant to serve as an in vitro representation of trans-epithelial effects of airway diesel and particulate matter exposures on fibroblasts in the stroma. The epithelial cells in the airway epithelium are represented by 16HBE cells and the fibroblasts are represented by either the IMR90 cell line or primary fibroblasts. Disclaimer: The information presented here do not necessarily represent Agency policy. Mention of trade names or commercial products does not constitute endorsement or recommendations for use.

Impact/Purpose

This is a protocol for the set-up and use of the trans-epithelial exposure model (TEEM) for both low- and high-throughput inhaled chemical research and testing. Making this protocol easily accessible to the broader scientific field will facilitate external use of the model in inhaled chemical toxicity testing and research, as well as independent validation of findings published by EPA authors.

Citation

Faber, S., N. McNabb, AND S. McCullough. Method for Tran-Epithelial Exposure Model (TEEM) - Epithelial Cells and Fibroblasts. Springer Nature, LONDON, UK, 2020. [DOI: 10.21203/rs.2.18675/v1]

DOI: Method for Tran-Epithelial Exposure Model (TEEM) - Epithelial Cells and Fibroblasts