Systematic Evidence Mapping of Genotoxicity Data for Chloroform Carcinogenicity
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Systematic evidence mapping (SEM) provides a transparent and methodologically rigorous approach for compiling the available evidence to assess potential human health hazards. The U.S. Environmental Protection Agency’s Integrated Risk Information System (IRIS) program is currently reassessing human health effects of chloroform. This reassessment, in addition to human and animal data, is focused on evaluating newly identified genotoxicity studies. As part of this evaluation, a literature search was conducted for chloroform genotoxicity studies consistent with defined Populations, Exposure, Comparators, and Outcomes (PECO) criteria. The studies obtained from the various databases were screened in SWIFT Review software using evidence stream filters (human, animal, and in vitro studies). Following title and abstract and full-text screening in DistillerSR, PECO-relevant studies underwent study quality evaluation using Health Assessment Workspace Collaborative (HAWC) software. Genotoxicity studies were identified from the in vivo studies initially categorized as meeting SEM or refined PECO criteria or from supplemental in vivo, in vitro, ex vivo, or in silico mechanistic information. Mechanistic studies assessing genotoxicity were screened and tagged according to the type of genotoxicity assays used, including, for example, gene mutation tests, chromosomal aberrations, the micronucleus assay, and DNA damage assays. The results of this systematic review process will be presented. Overall, this systematic evidence mapping provided a transparent approach for evaluation of genotoxicity data for chloroform. Disclaimer: The views expressed in this abstract are those of the author(s) and do not necessarily represent the views or the policies of the US EPA.