Isolation of Total RNA with the Zymo Quick-RNA Miniprep Kit

This protocol describes the use of the ZYMO Quick-RNA miniprep kit for the isolation and purification of total RNA from cells grown in culture.  The conditions described below were developed for the isolation of RNA from H441 and HULEC from the alveolar-capillary region exposure (ACRE) model.

Impact/Purpose

To make our protocol for isolating total RNA from different cell types in the alveolar-capillary regions exposure (ACRE) in vitro model publicly available.

Citation

Vitucci, E. AND S. McCullough. Isolation of Total RNA with the Zymo Quick-RNA Miniprep Kit. Springer Nature, LONDON, UK1, (2022). [DOI: 10.21203/rs.3.pex-2015/v1]

DOI: Isolation of Total RNA with the Zymo Quick-RNA Miniprep Kit