Gene Expression of Mouse Liver and Brain after 5-day Oral Exposure of Anatoxin-a

This subproduct is a gene count dataset generated by gene expression of mouse liver and brain tissue after exposure to 5 days of the freshwater cyanotoxin anatoxin-a. Male and female CD-1 mice were dosed with either vehicle (water), 2, 4, or 6 mg/kg (+)-anatoxin-a extracted and purified from a natural bloom (n=5/sex/dose). This tissue was collected 2 weeks after the final anatoxin dose to evaluate for residual effects. The tissue was analyzed by Biospyder Technologies which uses targeted sequencing with Temp-O-Seq.

Impact/Purpose

The purpose of generating this dataset was to identify possible dose-response endpoints in the development of no effect and lowest effect levels for repeated, nonlethal dosing of anatoxin-a. Gene expression is a sensitive measure of toxin effects and these tissues were collected 2 weeks after the final dose in order to identify potential residual effects as biomarkers for nonlethal and post-recovery anatoxin exposure. Male and female CD-1, 10 week old mice were given either 0, 2, 4, or 6 mg/kg oral anatoxin once daily for 5 consecutive days. Acute effects were observed, especially in the 4 and 6 mg/kg groups, which included weakness, gait changes, and decreased movement. These effects appeared within 5 minutes of dosing and resolved clinically by 30 minutes.

Citation

Jenkins-Hill, D., B. Ruto, C. Wood, AND J. Cullen. Gene Expression of Mouse Liver and Brain after 5-day Oral Exposure of Anatoxin-a. U.S. Environmental Protection Agency, Washington, DC, 2026. [DOI: 10.23719/d-sj4r]

DOI: Gene Expression of Mouse Liver and Brain after 5-day Oral Exposure of Anatoxin-a